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odyssey infrared fluorescent scanner  (LI-COR)


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    LI-COR odyssey infrared fluorescent scanner
    Odyssey Infrared Fluorescent Scanner, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 7420 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/odyssey+infrared+fluorescence+scanner/Odyssey+FC+Imaging+System/pmc13130404-157-29-33
    Average 99 stars, based on 7420 article reviews
    odyssey infrared fluorescent scanner - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Fluorescence:

    Article Title: YTHDF1 regulates YTHDF2 stability via m6A-dependent mechanisms in hepatocellular carcinoma: insights from in vitro , in vivo , and multi-cohort clinical studies
    Article Snippet: .. Finally, bands were seen using an Odyssey infrared fluorescence scanner (LICOR, Lincoln, NE, USA). ..

    Article Title: Total flavonoids of litchi Seed alleviates schistosomiasis liver fibrosis in mice by suppressing hepatic stellate cells activation and modulating the gut microbiomes.
    Article Snippet: Infection with Schistosoma japonicum (S. japonicum) is an important zoonotic parasitic disease that causes liver fibrosis in both human and domestic animals.. The activation of hepatic stellate cells (HSCs) is a crucial phase in the development of liver fibrosis, and inhibiting their activation can alleviate this progression.. Total flavonoids of litchi seed (TFL) is a naturally extracted drug, and modern pharmacological studies have shown its anti-fibrotic and liver-protective effects.

    Article Title: AMH regulates ovary size by counteracting the positive influence of clustered ovarian follicle growth.
    Article Snippet: Proteins were transferred to a 0.4-μm nitrocellulose membrane (Whatman) at 30 volts for 1 h on ice. ow nloaded from https://academ ic.oup.com /hum rep/advance-article/doi/10.1093/hum rep/deag022/8498885 by guest on 02 M arch 2026 Blotting membranes were blocked with Odyssey blocking reagent (Licor) for 30 min then were probed with 0.1 μg/ml primary antibody to the N-terminal fragment of AMH (R&D systems, Cat No. AF2748, RRID: AB_2226475) then with 1 μg/ml IRDye 680RD donkey anti-goat IgG antibody (Licor, Cat No. 926-68074, RRID: AB_10956736). .. Immunofluorescence was imaged on an Odyssey infrared fluorescence scanner (Licor). .. Aliquots (625 ng) of recombinant human AMH (produced in HEK293 cells by PX Therapeutics under contract to University of Otago, �20% proAMH, 80% AMHN, C) were combined with 130 μl of an isoelectric focusing (IEF) rehydration buffer [containing 7 mol/l urea, 2 mol/l thiourea, 2% w/v CHAPS, 50 mM DTT, 5 mmol/l TCEP (triscarboxyethylphosphine), to which 25 μl of IEF buffer concentrate (IPG buffer, GE Healthcare, Cytiva) was added per 0.5 ml of rehydration buffer, just prior to use], and the solution used to rehydrate overnight Immobiline DryStrip pH 3–10, 7 cm, linear gradient IEF strips (GE Healthcare).

    Article Title: Siglec-15 is a putative receptor for porcine epidemic diarrhea virus infection.
    Article Snippet: Sixty micrograms of protein were loaded for SDS-polyacrylamide gel electrophoresis, and the proteins were transferred to a polyvinylidene difluoride (PVDF) membrane (IPFL00010, Millipore, Massachusetts, USA). .. The PVDF membrane was blocked with a 5% bovine serum albumin (BSA) solution (V900933, Sigma-Aldrich, St. Louis, MO, USA) at room temperature for 2 h. Primary antibodies, including GAPDH (1:5000), β-actin (1:5000), anti-PEDV NP (1:1000), Siglec-15 (1:1000), His (1:500), Flag (1:500), and HA (1:500), were incubated with the samples at 4 °C for 12 h. Next, the sections were incubated with IRDye® 800CW donkey anti-mouse IgG secondary antibody (1:10,000) and IRDye® 800CW donkey anti-rabbit IgG secondary antibody (1:10,000) at room temperature for 2 h. The PVDF membrane was then visualized via a LICOR Odyssey infrared fluorescence scanner (LI-COR) after washing with TBST. ..

    Article Title: AMH regulates ovary size by counteracting ovarian follicle cluster effects
    Article Snippet: Blotting membranes were blocked with Odyssey blocking reagent (Licor) for 30 min then were probed with 0.1 μg/ml primary antibody to the N-terminal fragment of AMH (R&D systems, Cat# AF2748, RRID:AB_2226475) then with 1 μg/mL IRDye 680RD donkey anti-goat IgG antibody (Licor, Cat# 926-68074, RRID:AB_10956736). .. Immunofluorescence was imaged on an Odyssey infrared fluorescence scanner (Licor). .. Aliquots of recombinant human AMH (625 ng) were combined with 130 μL of an isoelectric focusing (IEF) rehydration buffer [containing 7 mol/L urea, 2 mol/L thiourea, 2%w/v CHAPS, 50 mM DTT, 5 mmol/L TCEP (triscarboxyethylphosphine), to which 25 μL of IEF buffer concentrate (IPG buffer, GE Healthcare, Cytiva) was added per 0.5 mL of rehydration buffer, just prior to use], and the solution used to rehydrate overnight Immobiline DryStrip pH 3-10, 7 cm, linear gradient IEF strips (GE Healthcare).

    Article Title: Noncanonical function of epigenetic reader YTHDF1 inhibits MASLD progression by maintaining peroxisomes and mitochondrial homeostasis.
    Article Snippet: After blocking the membranes with fast block solution (Epizyme), they were treated with primary antibodies. .. Finally, the membranes were treated with fluorescent secondary antibodies before being scanned with an Odyssey infrared fluorescence scanner (LICOR). .. The details of antibodies are presented in Supplementary Table 3. qRT–PCR RNA isolation kits (Vazyme, #R701) or TRIzol reagents (Thermo Fisher) were used to isolate total RNA from cells and tissues.

    Article Title: HMGB1 Expression Levels Correlate with Response to Immunotherapy in Non-Small Cell Lung Cancer
    Article Snippet: .. Membranes were then incubated with horseradish peroxidase-conjugated anti-rabbit and anti-mouse antibodies at room temperature for 2 h and scanned using an Odyssey infrared fluorescence scanner (LI-COR Biosciences, Lincoln, NE, USA). .. C57BL/6 male mice (20 ± 2 g, 5–6-month-old) were obtained from Jiangsu Huachuang Sino Pharmaceutical Technology Co., Ltd. (Taizhou, Jiangsu, China).

    Immunofluorescence:

    Article Title: AMH regulates ovary size by counteracting the positive influence of clustered ovarian follicle growth.
    Article Snippet: Proteins were transferred to a 0.4-μm nitrocellulose membrane (Whatman) at 30 volts for 1 h on ice. ow nloaded from https://academ ic.oup.com /hum rep/advance-article/doi/10.1093/hum rep/deag022/8498885 by guest on 02 M arch 2026 Blotting membranes were blocked with Odyssey blocking reagent (Licor) for 30 min then were probed with 0.1 μg/ml primary antibody to the N-terminal fragment of AMH (R&D systems, Cat No. AF2748, RRID: AB_2226475) then with 1 μg/ml IRDye 680RD donkey anti-goat IgG antibody (Licor, Cat No. 926-68074, RRID: AB_10956736). .. Immunofluorescence was imaged on an Odyssey infrared fluorescence scanner (Licor). .. Aliquots (625 ng) of recombinant human AMH (produced in HEK293 cells by PX Therapeutics under contract to University of Otago, �20% proAMH, 80% AMHN, C) were combined with 130 μl of an isoelectric focusing (IEF) rehydration buffer [containing 7 mol/l urea, 2 mol/l thiourea, 2% w/v CHAPS, 50 mM DTT, 5 mmol/l TCEP (triscarboxyethylphosphine), to which 25 μl of IEF buffer concentrate (IPG buffer, GE Healthcare, Cytiva) was added per 0.5 ml of rehydration buffer, just prior to use], and the solution used to rehydrate overnight Immobiline DryStrip pH 3–10, 7 cm, linear gradient IEF strips (GE Healthcare).

    Article Title: AMH regulates ovary size by counteracting ovarian follicle cluster effects
    Article Snippet: Blotting membranes were blocked with Odyssey blocking reagent (Licor) for 30 min then were probed with 0.1 μg/ml primary antibody to the N-terminal fragment of AMH (R&D systems, Cat# AF2748, RRID:AB_2226475) then with 1 μg/mL IRDye 680RD donkey anti-goat IgG antibody (Licor, Cat# 926-68074, RRID:AB_10956736). .. Immunofluorescence was imaged on an Odyssey infrared fluorescence scanner (Licor). .. Aliquots of recombinant human AMH (625 ng) were combined with 130 μL of an isoelectric focusing (IEF) rehydration buffer [containing 7 mol/L urea, 2 mol/L thiourea, 2%w/v CHAPS, 50 mM DTT, 5 mmol/L TCEP (triscarboxyethylphosphine), to which 25 μL of IEF buffer concentrate (IPG buffer, GE Healthcare, Cytiva) was added per 0.5 mL of rehydration buffer, just prior to use], and the solution used to rehydrate overnight Immobiline DryStrip pH 3-10, 7 cm, linear gradient IEF strips (GE Healthcare).

    Membrane:

    Article Title: Siglec-15 is a putative receptor for porcine epidemic diarrhea virus infection.
    Article Snippet: Sixty micrograms of protein were loaded for SDS-polyacrylamide gel electrophoresis, and the proteins were transferred to a polyvinylidene difluoride (PVDF) membrane (IPFL00010, Millipore, Massachusetts, USA). .. The PVDF membrane was blocked with a 5% bovine serum albumin (BSA) solution (V900933, Sigma-Aldrich, St. Louis, MO, USA) at room temperature for 2 h. Primary antibodies, including GAPDH (1:5000), β-actin (1:5000), anti-PEDV NP (1:1000), Siglec-15 (1:1000), His (1:500), Flag (1:500), and HA (1:500), were incubated with the samples at 4 °C for 12 h. Next, the sections were incubated with IRDye® 800CW donkey anti-mouse IgG secondary antibody (1:10,000) and IRDye® 800CW donkey anti-rabbit IgG secondary antibody (1:10,000) at room temperature for 2 h. The PVDF membrane was then visualized via a LICOR Odyssey infrared fluorescence scanner (LI-COR) after washing with TBST. ..

    Incubation:

    Article Title: Siglec-15 is a putative receptor for porcine epidemic diarrhea virus infection.
    Article Snippet: Sixty micrograms of protein were loaded for SDS-polyacrylamide gel electrophoresis, and the proteins were transferred to a polyvinylidene difluoride (PVDF) membrane (IPFL00010, Millipore, Massachusetts, USA). .. The PVDF membrane was blocked with a 5% bovine serum albumin (BSA) solution (V900933, Sigma-Aldrich, St. Louis, MO, USA) at room temperature for 2 h. Primary antibodies, including GAPDH (1:5000), β-actin (1:5000), anti-PEDV NP (1:1000), Siglec-15 (1:1000), His (1:500), Flag (1:500), and HA (1:500), were incubated with the samples at 4 °C for 12 h. Next, the sections were incubated with IRDye® 800CW donkey anti-mouse IgG secondary antibody (1:10,000) and IRDye® 800CW donkey anti-rabbit IgG secondary antibody (1:10,000) at room temperature for 2 h. The PVDF membrane was then visualized via a LICOR Odyssey infrared fluorescence scanner (LI-COR) after washing with TBST. ..

    Article Title: HMGB1 Expression Levels Correlate with Response to Immunotherapy in Non-Small Cell Lung Cancer
    Article Snippet: .. Membranes were then incubated with horseradish peroxidase-conjugated anti-rabbit and anti-mouse antibodies at room temperature for 2 h and scanned using an Odyssey infrared fluorescence scanner (LI-COR Biosciences, Lincoln, NE, USA). .. C57BL/6 male mice (20 ± 2 g, 5–6-month-old) were obtained from Jiangsu Huachuang Sino Pharmaceutical Technology Co., Ltd. (Taizhou, Jiangsu, China).

    other:

    Article Title: HMGB1 Expression Levels Correlate with Response to Immunotherapy in Non-Small Cell Lung Cancer
    Article Snippet: In vivo Experiments C57BL/6 male mice (20 ± 2 g, 5–6-month-old) were obtained from Jiangsu Huachuang Sino Pharmaceutical Technology Co., Ltd. (Taizhou, Jiangsu, China).

    Infrared:

    Article Title: Noncanonical function of epigenetic reader YTHDF1 inhibits MASLD progression by maintaining peroxisomes and mitochondrial homeostasis.
    Article Snippet: After blocking the membranes with fast block solution (Epizyme), they were treated with primary antibodies. .. Finally, the membranes were treated with fluorescent secondary antibodies before being scanned with an Odyssey infrared fluorescence scanner (LICOR). .. The details of antibodies are presented in Supplementary Table 3. qRT–PCR RNA isolation kits (Vazyme, #R701) or TRIzol reagents (Thermo Fisher) were used to isolate total RNA from cells and tissues.



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    LI-COR odyssey infrared laser fluorescence scanner
    (A) Schematic of the STIM1/ORAI1 complex using split Venus as a yellow signal reporter in a bimolecular <t>fluorescence</t> complementation (BiFC) assay. Yellow fluorescence indicates STIM1/ORAI1 interaction. The signal is higher after shear stress compared to the static condition in THP-1 cells. (B) Quantification of the Venus-positive BiFC STIM1/ORAI1 THP-1 cells under shear stress and static conditions. The shear stress condition shows 10.60%-±0.70% Venus-positive cells, while the static condition only has 1.80% ±0.20% Venus-positive cells. (C) STIM1-KD and ORAI1-KD cells have decreased shear stress-mediated IL8 expression as compared to control THP-1 cells, as quantified by qPCR. Replicates, n = 6. (D) SPTAN1-KD and RAF1-KD cells have decreased STIM1 and ORAI1 interaction under shear stress condition based on decreased split Venus STIM1 and ORAI1 signal. Replicates, n = 6. (E) Quantification of flow cytometry data, showing that STIM1-KD and ORAI1-KD cells have decreased shear-mediated cellular necrosis and increased cell survival. Replicates, n = 6. (F) Proposed mode of the cascade molecular reaction induced by shear stress. (G) FR180204 , an ERK inhibitor, decreased shear stress-mediated IL8 expression in CD14 + primary monocytes. (H) Quantification of flow cytometry data, displaying a decrease in shear stress-mediated necrotic cells following treatment with FR180204 in CD14 + primary monocytes.
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    (A) Schematic of the STIM1/ORAI1 complex using split Venus as a yellow signal reporter in a bimolecular <t>fluorescence</t> complementation (BiFC) assay. Yellow fluorescence indicates STIM1/ORAI1 interaction. The signal is higher after shear stress compared to the static condition in THP-1 cells. (B) Quantification of the Venus-positive BiFC STIM1/ORAI1 THP-1 cells under shear stress and static conditions. The shear stress condition shows 10.60%-±0.70% Venus-positive cells, while the static condition only has 1.80% ±0.20% Venus-positive cells. (C) STIM1-KD and ORAI1-KD cells have decreased shear stress-mediated IL8 expression as compared to control THP-1 cells, as quantified by qPCR. Replicates, n = 6. (D) SPTAN1-KD and RAF1-KD cells have decreased STIM1 and ORAI1 interaction under shear stress condition based on decreased split Venus STIM1 and ORAI1 signal. Replicates, n = 6. (E) Quantification of flow cytometry data, showing that STIM1-KD and ORAI1-KD cells have decreased shear-mediated cellular necrosis and increased cell survival. Replicates, n = 6. (F) Proposed mode of the cascade molecular reaction induced by shear stress. (G) FR180204 , an ERK inhibitor, decreased shear stress-mediated IL8 expression in CD14 + primary monocytes. (H) Quantification of flow cytometry data, displaying a decrease in shear stress-mediated necrotic cells following treatment with FR180204 in CD14 + primary monocytes.
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    LI-COR li cor odyssey infrared fluorescence scanner
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    Image Search Results


    (A) Schematic of the STIM1/ORAI1 complex using split Venus as a yellow signal reporter in a bimolecular fluorescence complementation (BiFC) assay. Yellow fluorescence indicates STIM1/ORAI1 interaction. The signal is higher after shear stress compared to the static condition in THP-1 cells. (B) Quantification of the Venus-positive BiFC STIM1/ORAI1 THP-1 cells under shear stress and static conditions. The shear stress condition shows 10.60%-±0.70% Venus-positive cells, while the static condition only has 1.80% ±0.20% Venus-positive cells. (C) STIM1-KD and ORAI1-KD cells have decreased shear stress-mediated IL8 expression as compared to control THP-1 cells, as quantified by qPCR. Replicates, n = 6. (D) SPTAN1-KD and RAF1-KD cells have decreased STIM1 and ORAI1 interaction under shear stress condition based on decreased split Venus STIM1 and ORAI1 signal. Replicates, n = 6. (E) Quantification of flow cytometry data, showing that STIM1-KD and ORAI1-KD cells have decreased shear-mediated cellular necrosis and increased cell survival. Replicates, n = 6. (F) Proposed mode of the cascade molecular reaction induced by shear stress. (G) FR180204 , an ERK inhibitor, decreased shear stress-mediated IL8 expression in CD14 + primary monocytes. (H) Quantification of flow cytometry data, displaying a decrease in shear stress-mediated necrotic cells following treatment with FR180204 in CD14 + primary monocytes.

    Journal: Cell reports

    Article Title: A shear stress-responsive pathway in monocytes drives cardiopulmonary bypass-induced inflammation via spectrin/RAF1/store-operated calcium entry

    doi: 10.1016/j.celrep.2025.116903

    Figure Lengend Snippet: (A) Schematic of the STIM1/ORAI1 complex using split Venus as a yellow signal reporter in a bimolecular fluorescence complementation (BiFC) assay. Yellow fluorescence indicates STIM1/ORAI1 interaction. The signal is higher after shear stress compared to the static condition in THP-1 cells. (B) Quantification of the Venus-positive BiFC STIM1/ORAI1 THP-1 cells under shear stress and static conditions. The shear stress condition shows 10.60%-±0.70% Venus-positive cells, while the static condition only has 1.80% ±0.20% Venus-positive cells. (C) STIM1-KD and ORAI1-KD cells have decreased shear stress-mediated IL8 expression as compared to control THP-1 cells, as quantified by qPCR. Replicates, n = 6. (D) SPTAN1-KD and RAF1-KD cells have decreased STIM1 and ORAI1 interaction under shear stress condition based on decreased split Venus STIM1 and ORAI1 signal. Replicates, n = 6. (E) Quantification of flow cytometry data, showing that STIM1-KD and ORAI1-KD cells have decreased shear-mediated cellular necrosis and increased cell survival. Replicates, n = 6. (F) Proposed mode of the cascade molecular reaction induced by shear stress. (G) FR180204 , an ERK inhibitor, decreased shear stress-mediated IL8 expression in CD14 + primary monocytes. (H) Quantification of flow cytometry data, displaying a decrease in shear stress-mediated necrotic cells following treatment with FR180204 in CD14 + primary monocytes.

    Article Snippet: Co-detection was performed using IRDye 700 and 800-labeled secondary antibodies (926–68070 and 926–32211, Li-Cor) under the Odyssey infrared laser fluorescence scanner (Li-Cor) and quantified using ImageJ.

    Techniques: Bimolecular Fluorescence Complementation Assay, Fluorescence, Shear, Expressing, Control, Flow Cytometry